瑞典卡罗琳斯卡大学Carmen Gerlach研究团队揭示，趋化因子受体CX3CR1的分级表达标志着人和小鼠T细胞的分化状态，并使跨物种解释成为可能。相关论文于2023年7月24日发表在《免疫》杂志上。

他们旨在鉴定反映CD8+ T细胞分化的分级性质的表面标记物，并描述小鼠和人类的功能可比较状态。CITEseq分析显示，编码趋化因子受体CX3CR1的CX3CR1的分级表达与CD8+ T细胞分化梯度相关。CX3CR1的表达区分人类和小鼠CD8+和CD4+ T细胞状态，通过迁移和功能特性来定义。CX3CR1的分级表达，加上CD62L的细化，准确地捕获了高维T细胞分化连续体。

此外，CX3CR1表达梯度描述了人类和小鼠在稳态和纵向追踪两种物种的病毒特异性CD8+ T细胞中的具有相似特性的状态。因此，渐变的CX3CR1表达提供了一种策略来翻译不同物种间T细胞分化状态的行为。

据介绍，T细胞在遇到抗原时分化成不同的功能状态。这些状态是由小鼠和人类T细胞的不同细胞表面标记物描述的，这阻碍了T细胞特性的跨物种翻译。

附：英文原文

Title: Graded expression of the chemokine receptor CX3CR1 marks differentiation states of human and murine T cells and enables cross-species interpretation

Author: Anthonie Johan Zwijnenburg, Jyoti Pokharel, Renata Varnait, Wenning Zheng, Elena Hoffer, Iman Shryki, Natalia Ramirez Comet, Marcus Ehrstrm, Sara Gredmark-Russ, Liv Eidsmo, Carmen Gerlach

Issue&Volume: 2023-07-24

Abstract: T cells differentiate into functionally distinct states upon antigen encounter. These states are delineated by different cell surface markers for murine and human T cells, which hamper cross-species translation of T cell properties. We aimed to identify surface markers that reflect the graded nature of CD8+ T cell differentiation and delineate functionally comparable states in mice and humans. CITEseq analyses revealed that graded expression of CX3CR1, encoding the chemokine receptor CX3CR1, correlated with the CD8+ T cell differentiation gradient. CX3CR1 expression distinguished human and murine CD8+ and CD4+ T cell states, as defined by migratory and functional properties. Graded CX3CR1 expression, refined with CD62L, accurately captured the high-dimensional T cell differentiation continuum. Furthermore, the CX3CR1 expression gradient delineated states with comparable properties in humans and mice in steady state and on longitudinally tracked virus-specific CD8+ T cells in both species. Thus, graded CX3CR1 expression provides a strategy to translate the behavior of distinct T cell differentiation states across species.

DOI: 10.1016/j.immuni.2023.06.025

Source: https://www.cell.com/immunity/fulltext/S1074-7613(23)00282-0