瑞典卡罗林斯卡学院Gunilla B. Karlsson Hedestam团队开发出恒河猴和食蟹猕猴的种系VDJ等位基因综合数据库。相关论文于2021年1月22日在线发表在《免疫》杂志上。

据研究人员介绍，定义个体中特定的种系免疫球蛋白（Ig）等位基因是解析抗原特异性抗体应答的发生和发展的第一步。恒河猴和食蟹猕猴是临床前研究的重要动物模型，主要分为四个亚组：印度和中国的恒河猴以及毛里求斯和印度尼西亚的食蟹猕猴。研究人员开发了Ig基因推断工具IgDiscover，并进行了广泛的基因组验证，从而定义了这4个亚组中的种系VDJ等位基因，总共包括45只猕猴。在中国和印度起源的恒河猴之间，以及在两个恒河猴物种之间，存在等位基因重叠。由于受岛屿限制，毛里求斯食蟹猕猴种群的亚群等位基因数量最少，但个体等位基因多样性却很高。 

这些关于恒河猴和食蟹猕猴的IGH等位基因综合基因库为重要临床前模型中的B细胞应答研究提供了资源。 

附：英文原文

Title: Rhesus and cynomolgus macaque immunoglobulin heavy-chain genotyping yields comprehensive databases of germline VDJ alleles

Author: Néstor Vázquez Bernat, Martin Corcoran, Izabela Nowak, Mateusz Kaduk, Xaquin Castro Dopico, Sanjana Narang, Pauline Maisonasse, Nathalie Dereuddre-Bosquet, Ben Murrell, Gunilla B. Karlsson Hedestam

Issue&Volume: 2021-01-22

Abstract: Definition of the specific germline immunoglobulin (Ig) alleles present in an individual is a critical first step to delineate the ontogeny and evolution of antigen-specific antibody responses. Rhesus and cynomolgus macaques are important animal models for pre-clinical studies, with four main sub-groups being used: Indian- and Chinese-origin rhesus macaques and Mauritian and Indonesian cynomolgus macaques. We applied the (Ig) gene inference tool IgDiscover and performed extensive Sanger sequencing-based genomic validation to define germline VDJ alleles in these 4 sub-groups, comprising 45 macaques in total. There was allelic overlap between Chinese- and Indian-origin rhesus macaques and also between the two macaque species, which is consistent with substantial admixture. The island-restricted Mauritian cynomolgus population displayed the lowest number of alleles of the sub-groups, yet maintained high individual allelic diversity. These comprehensive databases of germline IGH alleles for rhesus and cynomolgus macaques provide a resource toward the study of B cell responses in these important pre-clinical models.

DOI: 10.1016/j.immuni.2020.12.018

Source: https://www.cell.com/immunity/fulltext/S1074-7613(20)30546-X

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