近日，东南大学彭汉川课题组实现小鼠全脑的跨模式相干配准。相关论文发表在2021年12月9日在线发表在《自然—方法学》杂志上。

研究人员报道了一种跨模式的注册方法——mBrainAligner，它使用相干的地标映射和深度神经网络将小鼠全脑图像与标准的Allen共同坐标框架图集对齐。研究人员为荧光显微光学切片断层成像模式建立了一个脑图谱，以促进单细胞绘图，并使用这个方法生成了一个三维单神经元形态和神经元细胞类型的全脑图谱。

据了解，最近的全脑图谱项目正在收集大规模的三维图像，使用的方式包括：串行双光子断层扫描、荧光微光切片断层扫描、光片荧光显微镜、带有同步即时扫描和读出的容积成像或磁共振成像。将这些多维全脑图像注册到标准图谱上，对描述神经元类型和构建大脑线路图至关重要。然而，由于大脑解剖学的内在变化以及不同的样品制备方法和成像模式导致的伪影，跨模式的图像注册是具有挑战性的。

附：英文原文

Title: Cross-modal coherent registration of whole mouse brains

Author: Qu, Lei, Li, Yuanyuan, Xie, Peng, Liu, Lijuan, Wang, Yimin, Wu, Jun, Liu, Yu, Wang, Tao, Li, Longfei, Guo, Kaixuan, Wan, Wan, Ouyang, Lei, Xiong, Feng, Kolstad, Anna C., Wu, Zhuhao, Xu, Fang, Zheng, Yefeng, Gong, Hui, Luo, Qingming, Bi, Guoqiang, Dong, Hongwei, Hawrylycz, Michael, Zeng, Hongkui, Peng, Hanchuan

Issue&Volume: 2021-12-09

Abstract: Recent whole-brain mapping projects are collecting large-scale three-dimensional images using modalities such as serial two-photon tomography, fluorescence micro-optical sectioning tomography, light-sheet fluorescence microscopy, volumetric imaging with synchronous on-the-fly scan and readout or magnetic resonance imaging. Registration of these multi-dimensional whole-brain images onto a standard atlas is essential for characterizing neuron types and constructing brain wiring diagrams. However, cross-modal image registration is challenging due to intrinsic variations of brain anatomy and artifacts resulting from different sample preparation methods and imaging modalities. We introduce a cross-modal registration method, mBrainAligner, which uses coherent landmark mapping and deep neural networks to align whole mouse brain images to the standard Allen Common Coordinate Framework atlas. We build a brain atlas for the fluorescence micro-optical sectioning tomography modality to facilitate single-cell mapping, and used our method to generate a whole-brain map of three-dimensional single-neuron morphology and neuron cell types. mBrainAligner is a cross-modal registration platform for whole mouse brains imaged with different modalities. In addition, a fluorescence micro-optical sectioning tomography-based mouse brain atlas has been generated.

DOI: 10.1038/s41592-021-01334-w

Source: https://www.nature.com/articles/s41592-021-01334-w

期刊信息

Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：28.467

官方网址：https://www.nature.com/nmeth/

投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex